bySaayman, Sheena; Arbuthnot, Patrick; Weinberg, Marc S.
This article is from Nucleic Acids Research, volume 38.AbstractSeveral different approaches exist to generate expressed RNA interference (RNAi) precursors for multiple target inhibition, a strategy referred to as combinatorial (co)RNAi. One such approach makes use of RNA Pol III-expressed long hairpin RNAs (lhRNAs), which are processed by Dicer to generate multiple unique short interfering siRNA effectors. However, because of inefficient intracellular Dicer processing, lhRNA duplexes have been... Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2965221
bySibley, Christopher R.; Seow, Yiqi; Saayman, Sheena; Dijkstra, Krijn K.; El Andaloussi, Samir; Weinberg, Marc S.; Wood, Matthew J. A.
This article is from Nucleic Acids Research, volume 40.AbstractMirtrons, short hairpin pre-microRNA (miRNA) mimics directly produced by intronic splicing, have recently been identified and experimentally confirmed in invertebrates. While there is evidence to suggest several mammalian miRNAs have mirtron origins, this has yet to be experimentally demonstrated. Here, we characterize the biogenesis of mammalian mirtrons by ectopic expression of splicing-dependent mirtron precursors. The putative... Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3245937